DNA Purification By Silica Membrane Spin Column

Sodium iodide solution (NaI, 6M)

Product specification: 100ml/200ml

Product introduction:

The molecular weight of sodium iodide is 149.89 and the chemical formula is NaI. Sodium iodide solution (NaI, 6M) can be used to purify DNA by silica membrane spin column method. This solution is quick, convenient and high in purity.

product composition:

Operation steps (for reference only):

1. Add 3 volumes of NaI (6M) to a 1.5ml centrifuge tube containing DNA solution and mix well.

2. Add the above solution to the silica membrane spin column, centrifuge at 14000g for 1 min, separate the spin column and the collection tube, discard the liquid in the collection tube, and reassemble the spin column and collection tube.

3. Add 750 μl of washing solution, centrifuge as above, and discard the liquid in the collection tube.

4. Centrifuge at 14,000g for 1 min to remove residual wash solution.

5. Transfer the spin column to a 1.5ml microcentrifuge tube, add 50-100μl sterile water or TE to the middle of the membrane, and leave at room temperature for 2-10min.

6. Centrifuge at 14000g for 1 min to collect the DNA solution.

Precautions:

1. If the amount used each time is very small, it can be appropriately divided before use.

2. Discard if precipitate occurs.

3. For your safety and health, please wear a lab coat and disposable gloves.

Validity: Valid for 3 months.

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